High frame rate. High sensitivity.
Bliq Photonics’ Video-rate Microscopy System (VMS) is a robust and flexible laser-scanning microscope built for speed, precision and versatility. The VMS features a high-speed polygonal scanner coupled with sensitive detectors, with a video-rate acquisition speed of 30 FPS (frames per second) at 1024 by 512 pixels and up to 480 FPS at 1024 by 32 pixels. These extreme scanning speeds are ideal for in vivo imaging applications that require high temporal resolution such as calcium imaging, optogenetics and blood cell behavior. As opposed to resonant scanners, the VMS’ polygonal scanner provides a constant linear scan and superior precision of laser positioning offering a larger field of view. With up to 9 sensitive MultiAlkali PMT detectors, you will not miss any relevant information. Additionally, each VMS comes with a reflectance APD detector that provides extra structural information about your sample.
Bliq Photonics’ VMS is built for both speed and sensitivity. The VMS is therefore perfect for imaging fast and dynamic events (in vivo and ex vivo) as well as signal coming from a continuously moving animal or tissue. There is a wide array of applications for this system.
- Ideal for imaging fast and dynamic events occurring in vivo and ex vivo, including :
- Calcium imaging
- Cell behavior dynamic
- Migration under flow
- Compatible with label-free techniques, including :
- Coherent Anti-Stokes Raman Scattering (CARS)
- Second Harmonic Generation (SHG)
- Third Harmonic Generation (THG)
- Super-resolution microscopy using Bliq Photonics’ SLAM module;
- Rapid volumetric imaging using Bliq Photonics’ Axicon module.
- Other applications include (but are not limited to):
- Photo-stimulation (visible and/or IR light)
- Patch clamp
- Photon counting
- Cell tacking
- Multiphoton/confocal two-in-one system
It’s not just an imaging system, it’s your tailor-made and personalized microscope.
Fully customized, zero compromise.
Bliq’s approach to imaging system customization is simple; our engineering team helps you efficiently design the system that you need so you don’t have to pay for unnecessary components. Here are a few examples of Bliq Photonics’ custom solutions :
- Multiphoton VMS with both visible and two-photon photo-stimulation;
- Multiphoton VMS with custom phosphorescence lifetime measurement;
- Axicon integration into a commercial multiphoton system;
- Video-microscope for live imaging with 2 simultaneous widefield illuminations (100 FPS);
- Custom mouse holder for brain imaging.
Specifications – VMS*
|Light sources||Infrared pulsed laser||MaiTai series from Spectra-Physics|
|Insight series from Spectra-Physics|
|Chameleon series from Coherent|
|Discovery series from Coherent|
|Laser power control||Polarization, AOM or EOM attenuation|
|Visible laser combiner||Up to 7 different laser lines|
|Epifluorescence||All modern lamp sources (LED or not)|
|Scanning||Polygonal scanner + galvanometer||X axis: 9 – 18 kHz (lines per second); Y axis: galvanometer mirror|
|Frame rate||30 FPS at 1 024 x 512, 480 FPS at 1 024 x 32|
|Light path||Gold mirrors (<4% power loss)|
|Broadband dielectric mirrors (<1% power loss)|
|Scanning options||XY, XYZ, XYT, XYZT|
|Detection||High-sensitivity detectors (PMTs)||Up to 3 MultiAlkali Photomultiplier Tubes (up to 9 on custom systems)|
|Analog and digital output|
|Reflectance detector||APD reflectance detector|
|Micoscope||Stand||Upright and inverted|
|Computer||Hardware||4.2 GHz, 128 GB SDRAM, 4 TB SSD, 27″ 5K display (5120 by 2880), Thunderbolt 3 (40 Go/s)|
|Software||Base module||X and Y axis scanning control|
|PMT gain and tension control|
|Z axis movement control|
|Motorized stage module||XYZT (tiling, multi-site acquisition)|
|Photo-stimulation module||Multi-point, scanned multi-ROI and flashed multi-ROI|
|Triggers for stimulation and acquisition|
|Axicon control module||Volumetric imaging including bypass mode|
|SLAM control module||Automated control of the super-resolution module|
Sensory Afferents Use Different Coding Strategies for Heat and Cold. (2018) Feng Wang, Erik Bélanger, Sylvain L. Coté, Patrick Desrosiers, Steven A. Prescott, Daniel C. Côté, Yves De Koninck. Cell Reports, Vol. 23, No. 7, May 15, 2001-2013. (PubMed)
In Vivo Cell Tracking With Video Rate Multimodality Laser Scanning Microscopy. (2008) Israel Veilleux, Joel A. Spencer, David P. Biss, Daniel Côté, and Charles P. Lin. IEEE Journal Of Selected Topics In Quantum Electronics, Vol. 14, No. 1, January/February. (IEEE Xplore)