Resources – Bliq Photonics

ALL SOLUTIONS

Bliq solutions overview

Download the brochure

LIGHTSHEET

How does the Bliq 2P Bessel light sheet system achieve high resolution images of large specimens?

Nafiseh Rafiei, Alicja Gasecka, and Marieve Picard

Download the white paper

Light sheet microscopy to study the development of dopaminergic circuits

The laboratory of Martin Lévesque at the CERVO Research Center

Download the case study

VMS

In Vivo Cell Tracking With Video Rate Multimodality Laser Scanning Microscopy

Israel Veilleux; Joel A. Spencer; David P. Biss; Daniel Cote; Charles P. Lin

IEEE Journal of Selected Topics in Quantum Electronics – 14: 10-18, 2008

https://ieeexplore.ieee.org/document/4451134

Association of a History of Child Abuse With Impaired Myelination in the Anterior Cingulate Cortex: Convergent Epigenetic, Transcriptional, and Morphological Evidence

Pierre-Eric Lutz, Arnaud Tanti, Alicja Gasecka, Sarah Barnett-Burns, John J Kim, Yi Zhou, Gang G Chen, Marina Wakid, Meghan Shaw, Daniel Almeida, Marc-Aurele Chay, Jennie Yang, Vanessa Larivière, Marie-Noël M’Boutchou, Léon C van Kempen, Volodymyr Yerko, Josée Prud’homme, Maria Antonietta Davoli, Kathryn Vaillancourt, Jean-François Théroux, Alexandre Bramoullé, Tie-Yuan Zhang, Michael J Meaney, Carl Ernst, Daniel Côté, Naguib Mechawar, Gustavo Turecki

American Journal of Psychiatry – 174: 1185-1194, 2017

https://ajp.psychiatryonline.org/doi/10.1176/appi.ajp.2017.16111286

Intravital assessment of myelin molecular order with polarimetric multiphoton microscopy

Turcotte, R., Rutledge, D.J., Bélanger, E., Dill, D., Macklin, W.B., Côté, D.C.

Scientific Reports – 6 (1) : 31685, 2016

https://www.nature.com/articles/srep31685

Sensory Afferents Use Different Coding Strategies for Heat and Cold

Feng Wang, Erik Bélanger, Sylvain L. Côté , Patrick Desrosiers, Steven A. Prescott, Daniel C. Côté, Yves De Koninck

Cell Reports – 23: 2001–2013, 2018

https://www.cell.com/cell-reports/pdf/S2211-1247%2818%2930624-7.pdf

SPARQ

Optical sectioning microscopy with planar or structured illumination

Jerome Mertz

Nature Methods – 8: 811-819, 2011

https://www.nature.com/articles/nmeth.1709

Optically sectioned in vivo imaging with speckle illumination HiLo microscopy

Daryl Lim, Tim N Ford, Kengyeh K Chu, Jerome Mertz

Journal of Biomedical Optics – 16: 016014, 2011

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055591/

Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy

Daryl Lim, Kengyeh K. Chu, and Jerome Mertz

Optics Letters – 33: 1819-1821, 2008

https://www.osapublishing.org/ol/viewmedia.cfm?uri=ol-33-16-1819&seq=0

AXICON

Extended depth of field microscopy for rapid volumetric two-photon imaging

Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy

Optics Express – 21 (8): 10095, 2013

https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-21-8-10095&id=252855

SLAM

Resolution and contrast enhancement in laser scanning microscopy using dark beam imaging

Harold Dehez, Michel Piché, and Yves De Koninck

Optique Express- 21: 15912, 2013

https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-21-13-15912&id=258317

Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

Alicja Gasecka, Amy Daradich, Harold Dehez, Michel Piché, and Daniel Côté

Optique Letters – 38 (21): 4510, 2013

https://www.osapublishing.org/ol/fulltext.cfm?uri=ol-38-21-4510&id=273987

Applications	Features	Benefits
Fixed cell imaging	- Multi-channel acquisition	SPARQ can be used with any fluorophore
Live cell imaging	- Low laser intensity (comparable to standard widefield illumination)	Reduces photobleaching and phototoxity, allowing for long imaging sessions
	- Fast 2D and 3D imaging	With a frame rate of up to 10 FPS, catch your dynamic proccesses using SPARQ
	- Multi-dimensional acquisition: Time lapse, z-stacks, multi-channels	Set your usual multi-dimensional experiments and turn on SPARQ for optically sectioned images
	- Combinaison with transmitted light constrat methods	Combine your SPARQ images with any contrast modes, without even changing objectives
Tissue slice imaging	- High penetration depth	Since the speckles remain sharp in depth, get the same optical sectioning capabilities as you go deeper
	- Modification of optical thickness	Adjust SPARQ's thickness factor parameter to change the axial resolution without affecting the lateral resolution
	- 3D imaging and reconstruction	Create high quality 3D images by rejecting out-of-focus fluorescence
Whole mount and cleared tissue imaging	- Large area stitching	Keep the same imaging speed regardless of the field of view. SPARQ works with any camera
	- Use different objective magnifications	Simply change objectives without the need to re-calibrate or to modify the SPARQ parameter
	- Works with any kind of objectives	Use high N.A, LWD or phase contrast objectives. It will not affect the SPARQ images
	- Keep high axial and lateral resolution	Improve the optical sectioning without sacrificing the lateral resolution as with standard confocal microscopy
In vivo imaging (on 2P)	- Can be used in combinaison with photo-stimulation	Use the same visible lasers to photo-stimulate and do SPARQ imaging (different light paths)
	- Offers optical sectioning with visible lasers on a 2P optimized system	Increase the flexibilty of your 2P system by adding SPARQ and get visible light confocality
	- Can be used in vivo for positioning and other fluorescence uses	A one push button allows you to easily switch between SPARQ and other imaging modes

Publications