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  • Speed Running Your Sample: Faster Laser Scanning in Confocal and Multiphoton Microscopes
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    Applications

    Applications

    Features

    Benefits

    Fixed cell imaging

    - Multi-channel acquisitionSPARQ can be used with any fluorophore

    Live cell imaging

    - Low laser intensity (comparable to standard widefield illumination)Reduces photobleaching and phototoxity, allowing for long imaging sessions
    - Fast 2D and 3D imaging With a frame rate of up to 10 FPS, catch your dynamic proccesses using SPARQ
    - Multi-dimensional acquisition: Time lapse, z-stacks, multi-channelsSet your usual multi-dimensional experiments and turn on SPARQ for optically sectioned images
    - Combinaison with transmitted light constrat methodsCombine your SPARQ images with any contrast modes, without even changing objectives

    Tissue slice imaging



    - High penetration depthSince the speckles remain sharp in depth, get the same optical sectioning capabilities as you go deeper
    - Modification of optical thickness Adjust SPARQ's thickness factor parameter to change the axial resolution without affecting the lateral resolution
    - 3D imaging and reconstructionCreate high quality 3D images by rejecting out-of-focus fluorescence

    Whole mount and cleared tissue imaging

    - Large area stitchingKeep the same imaging speed regardless of the field of view. SPARQ works with any camera
    - Use different objective magnifications Simply change objectives without the need to re-calibrate or to modify the SPARQ parameter
    - Works with any kind of objectivesUse high N.A, LWD or phase contrast objectives. It will not affect the SPARQ images
    - Keep high axial and lateral resolutionImprove the optical sectioning without sacrificing the lateral resolution as with standard confocal microscopy

    In vivo imaging (on 2P)

    - Can be used in combinaison with photo-stimulationUse the same visible lasers to photo-stimulate and do SPARQ imaging (different light paths)
    - Offers optical sectioning with visible lasers on a 2P optimized systemIncrease the flexibilty of your 2P system by adding SPARQ and get visible light confocality
    - Can be used in vivo for positioning and other fluorescence usesA one push button allows you to easily switch between SPARQ and other imaging modes
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