Notes de terrain sur la microscopie

In our previous post, we discussed how naturally occurring fluorescence, or “autofluorescence” is present in many samples. This inherent signal can sometimes interfere with the desired fluorescence emission from applied probes, and is often discarded as “background”....

Some biological samples exhibit naturally occurring fluorescence, even in the absence of applied labels. This type of fluorescence is known as autofluorescence, and arises from naturally-occuring fluorophores such as collagen, lipofuscin, and flavins (just to name a...

High-speed multiphoton (MP) imaging is often used to visualize dynamic changes in live tissue or cellular preparations. For neuroscience applications, electrophysiology often is combined with imaging, and allows for the simultaneous acquisition and/or control of...

It is useful to have quantitative measures of image quality, so that you can systematically determine the effect of changing different imaging conditions on experiment results. In the last post we showed that averaging can improve image quality when carrying out...

In our previous blog post we discussed resonant and polygonal mirror scanning systems. These scanning systems are designed for high-speed imaging, and can achieve short pixel dwell times compared to conventional galvanometer mirrors. While short pixel dwell times...

In our previous post, we discussed galvo scanning systems used in laser scanning confocal and multiphoton microscopes. Galvo scanning is standard, yet for some applications, such as live-cell and large-format image acquisitions, systems that offer increased...