Pina Colarusso and Craig Brideau

Pina has been working as an optical microscopist over the past twenty years. Currently she directs an imaging centre, the Snyder Institute’s Live Imaging Laboratory (LCI) at the University of Calgary.

Author articles

Navigating the Variable Landscape of Antibody Selection

Navigating the Variable Landscape of Antibody Selection

Antibodies play a crucial role in life science research and are standard reagents in various imaging applications. In 2022, the global market for research antibodies was valued at approximately $4 billion USD, and it is projected to reach $6.5 billion USD by 2032....

Autofluorescence as an Aid to Discovery

Autofluorescence as an Aid to Discovery

In our previous post, we discussed how naturally occurring fluorescence, or “autofluorescence” is present in many samples. This inherent signal can sometimes interfere with the desired fluorescence emission from applied probes, and is often discarded as “background”....

An Important Yet Neglected Presence: Autofluorescence

An Important Yet Neglected Presence: Autofluorescence

Some biological samples exhibit naturally occurring fluorescence, even in the absence of applied labels. This type of fluorescence is known as autofluorescence, and arises from naturally-occuring fluorophores such as collagen, lipofuscin, and flavins (just to name a...

Twice the Challenge, but Double the Opportunity

Twice the Challenge, but Double the Opportunity

High-speed multiphoton (MP) imaging is often used to visualize dynamic changes in live tissue or cellular preparations. For neuroscience applications, electrophysiology often is combined with imaging, and allows for the simultaneous acquisition and/or control of...

Mind your Q’s: Quantifying image quality

Mind your Q’s: Quantifying image quality

It is useful to have quantitative measures of image quality, so that you can systematically determine the effect of changing different imaging conditions on experiment results. In the last post we showed that averaging can improve image quality when carrying out...

Unraveling Noise

Unraveling Noise

In our previous blog post we discussed resonant and polygonal mirror scanning systems. These scanning systems are designed for high-speed imaging, and can achieve short pixel dwell times compared to conventional galvanometer mirrors. While short pixel dwell times...

Powering Through It: Imaging Deep in Scattering Media

Powering Through It: Imaging Deep in Scattering Media

In the last post, we reviewed how scattering and absorption attenuate the intensity of the laser used to excite fluorescence and reduce the amount of emission signal that can make it to the detector(s). Tissue is very complex, and fluorescence from different dyes, as...

Friends and Foes: Scattering and Absorption

Friends and Foes: Scattering and Absorption

When carrying out fluorescence imaging in thicker samples, we often hear about how  “scattering” affects image quality by limiting the depth of imaging within the sample. Scattering occurs when light traveling through a sample encounters a change in refractive index, ...

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