Pina Colarusso and Craig Brideau

Pina has been working as an optical microscopist over the past twenty years. Currently she directs an imaging centre, the Snyder Institute’s Live Imaging Laboratory (LCI) at the University of Calgary.

Author articles

Practical Advice for Acquiring Multiphoton Lasers

Practical Advice for Acquiring Multiphoton Lasers

In our previous posts in this series, we discussed the broad range of excitation wavelengths for 2P fluorescence and described how many fluorophores can be simultaneously excited by a single excitation wavelength. We have covered the basics of two-photon microscopy...

A Dash of Photophysics Part 2: Absorbing the nuances of 2P spectra

A Dash of Photophysics Part 2: Absorbing the nuances of 2P spectra

With the advent of high-powered, pulsed lasers, two-photon (2P) microscopy is now a routine technique for imaging biological samples. As described in earlier posts, 2P microscopy is ideally suited for thick and/or scattering samples, such as organoids, tissue slices,...

A Dash of Photophysics Part 1: 2P vs 1P Fluorophore Excitation

A Dash of Photophysics Part 1: 2P vs 1P Fluorophore Excitation

When mapping out a fluorescence imaging experiment, it is important to find the optimal probe(s) for your application. When choosing a probe, a major consideration is matching the excitation and emission spectra to your microscope’s settings. For wide-field and...

Navigating the Variable Landscape of Antibody Selection

Navigating the Variable Landscape of Antibody Selection

Antibodies play a crucial role in life science research and are standard reagents in various imaging applications. In 2022, the global market for research antibodies was valued at approximately $4 billion USD, and it is projected to reach $6.5 billion USD by 2032....

Autofluorescence as an Aid to Discovery

Autofluorescence as an Aid to Discovery

In our previous post, we discussed how naturally occurring fluorescence, or “autofluorescence” is present in many samples. This inherent signal can sometimes interfere with the desired fluorescence emission from applied probes, and is often discarded as “background”....

An Important Yet Neglected Presence: Autofluorescence

An Important Yet Neglected Presence: Autofluorescence

Some biological samples exhibit naturally occurring fluorescence, even in the absence of applied labels. This type of fluorescence is known as autofluorescence, and arises from naturally-occuring fluorophores such as collagen, lipofuscin, and flavins (just to name a...

Twice the Challenge, but Double the Opportunity

Twice the Challenge, but Double the Opportunity

High-speed multiphoton (MP) imaging is often used to visualize dynamic changes in live tissue or cellular preparations. For neuroscience applications, electrophysiology often is combined with imaging, and allows for the simultaneous acquisition and/or control of...

Mind your Q’s: Quantifying image quality

Mind your Q’s: Quantifying image quality

It is useful to have quantitative measures of image quality, so that you can systematically determine the effect of changing different imaging conditions on experiment results. In the last post we showed that averaging can improve image quality when carrying out...

Unraveling Noise

Unraveling Noise

In our previous blog post we discussed resonant and polygonal mirror scanning systems. These scanning systems are designed for high-speed imaging, and can achieve short pixel dwell times compared to conventional galvanometer mirrors. While short pixel dwell times...

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