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Photons to Pixels: Illuminating Bit Depth

Photons to Pixels: Illuminating Bit Depth

by Pina Colarusso and Craig Brideau | Aug 3, 2022 | Uncategorized

The meaning and relevance of bit depth is a common point of confusion for life scientists who use microscopes. Typically, when we mention bit depth in microscopy, we are referring to gradations in the intensity readings from a detector.  Let’s use a photomultiplier...
Two-Photon Microscopy: How to Minimize Optical Aberrations

Two-Photon Microscopy: How to Minimize Optical Aberrations

by Pina Colarusso and Craig Brideau | Jun 10, 2022 | Uncategorized

The main advantage of two-photon microscopy is the ability to image deeper into scattering samples, such as tissue and live specimens. One way that two-photon objectives assist deep imaging is by providing extended working distances compared to lenses for other...
Two-Photon Microscopy Objectives: Focusing on the Essentials

Two-Photon Microscopy Objectives: Focusing on the Essentials

by Pina Colarusso and Craig Brideau | May 19, 2022 | Uncategorized

In our last post, Craig and I introduced the basics of two-photon microscopy. This month we will review some considerations for choosing the best objective for your multiphoton imaging. If you are new to objectives and their properties, we recommend that you consult...
What Is Two-Photon Microscopy?

What Is Two-Photon Microscopy?

by Pina Colarusso and Craig Brideau | Apr 14, 2022 | Uncategorized

This week I am joined by Dr. Craig Brideau, an engineering scientist who has recently joined the LCI team. Craig has designed and built numerous optical systems, and has particular expertise in biomedical imaging using non-linear optics. Over the next several posts,...
Honouring Maria Goeppert-Mayer for International Women’s Day

Honouring Maria Goeppert-Mayer for International Women’s Day

by Pina Colarusso and Craig Brideau | Mar 8, 2022 | Uncategorized

In recognition of International Women’s Day, I would like to highlight the German-American physicist Maria Goeppert-Mayer (1906-1972). Goeppert-Mayer started off in mathematics but was inspired to switch to physics after attending a quantum mechanics seminar presented...
Multicolour Imaging: Moving From Theory to Practice

Multicolour Imaging: Moving From Theory to Practice

by Pina Colarusso and Craig Brideau | Mar 1, 2022 | Uncategorized

Multicolour fluorescence imaging, when rigorously applied, allows the selective visualization of two or more features within a sample. The qualification “when rigorously applied” is so important because multicolour imaging is prone to artifacts. For multicolour...
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  • Speed Running Your Sample: Faster Laser Scanning in Confocal and Multiphoton Microscopes
  • Connecting the Dots: Laser Scanning in Confocal and Multiphoton Microscopes
  • Powering Through It: Imaging Deep in Scattering Media
  • Friends and Foes: Scattering and Absorption
  • Highlighting Color Correction

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    Applications

    Applications

    Features

    Benefits

    Fixed cell imaging

    - Multi-channel acquisitionSPARQ can be used with any fluorophore

    Live cell imaging

    - Low laser intensity (comparable to standard widefield illumination)Reduces photobleaching and phototoxity, allowing for long imaging sessions
    - Fast 2D and 3D imaging With a frame rate of up to 10 FPS, catch your dynamic proccesses using SPARQ
    - Multi-dimensional acquisition: Time lapse, z-stacks, multi-channelsSet your usual multi-dimensional experiments and turn on SPARQ for optically sectioned images
    - Combinaison with transmitted light constrat methodsCombine your SPARQ images with any contrast modes, without even changing objectives

    Tissue slice imaging



    - High penetration depthSince the speckles remain sharp in depth, get the same optical sectioning capabilities as you go deeper
    - Modification of optical thickness Adjust SPARQ's thickness factor parameter to change the axial resolution without affecting the lateral resolution
    - 3D imaging and reconstructionCreate high quality 3D images by rejecting out-of-focus fluorescence

    Whole mount and cleared tissue imaging

    - Large area stitchingKeep the same imaging speed regardless of the field of view. SPARQ works with any camera
    - Use different objective magnifications Simply change objectives without the need to re-calibrate or to modify the SPARQ parameter
    - Works with any kind of objectivesUse high N.A, LWD or phase contrast objectives. It will not affect the SPARQ images
    - Keep high axial and lateral resolutionImprove the optical sectioning without sacrificing the lateral resolution as with standard confocal microscopy

    In vivo imaging (on 2P)

    - Can be used in combinaison with photo-stimulationUse the same visible lasers to photo-stimulate and do SPARQ imaging (different light paths)
    - Offers optical sectioning with visible lasers on a 2P optimized systemIncrease the flexibilty of your 2P system by adding SPARQ and get visible light confocality
    - Can be used in vivo for positioning and other fluorescence usesA one push button allows you to easily switch between SPARQ and other imaging modes
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