Microscopy Field Notes

A blog created to help life science researchers navigate the inspiring world of optical microscopy

Decoding and Demystifying Filters

Decoding and Demystifying Filters

Developing expertise in microscopy, like for any technical field, requires mastering fundamental concepts and skills while also developing fluency in the specific jargon. Although jargon can be convenient for facilitating communication, it often creates barriers for...

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The Path Is Made by Walking

The Path Is Made by Walking

This month we share some reflections from Grace Baruta, who is a PhD candidate in the Hirota Laboratory at the University of Calgary. I have known Grace since she was a summer student, and she has always taken the time to research options and map out an approach, from...

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The Sample Sets the Stage

The Sample Sets the Stage

So far, we’ve touched on the mindset, planning, and decisions required when carrying out imaging experiments. To highlight this framework with a real-life example, I have invited Louise Neave to share some of her experiences as a researcher new to imaging. Louise is a...

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The Imaging Experiment: First Steps

The Imaging Experiment: First Steps

Building on the last post, let’s focus on the initial steps of the experimental workflow and how to transfer these concepts into practice. Your research aims are the foundation, and they will guide your choice of model and define the type of information needed from...

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