Microscopy Field Notes
A blog created to help life science researchers navigate the inspiring world of optical microscopy
Multicolour Imaging: Moving From Theory to Practice
Multicolour fluorescence imaging, when rigorously applied, allows the selective visualization of two or more features within a sample. The qualification “when rigorously applied” is so important because multicolour imaging is prone to artifacts. For multicolour...
Decoding and Demystifying Filters
Developing expertise in microscopy, like for any technical field, requires mastering fundamental concepts and skills while also developing fluency in the specific jargon. Although jargon can be convenient for facilitating communication, it often creates barriers for...
The Path Is Made by Walking
This month we share some reflections from Grace Baruta, who is a PhD candidate in the Hirota Laboratory at the University of Calgary. I have known Grace since she was a summer student, and she has always taken the time to research options and map out an approach, from...
The Sample Sets the Stage
So far, we’ve touched on the mindset, planning, and decisions required when carrying out imaging experiments. To highlight this framework with a real-life example, I have invited Louise Neave to share some of her experiences as a researcher new to imaging. Louise is a...
The Imaging Experiment: First Steps
Building on the last post, let’s focus on the initial steps of the experimental workflow and how to transfer these concepts into practice. Your research aims are the foundation, and they will guide your choice of model and define the type of information needed from...
The Imaging Experiment: More Than Pretty Pictures
Though looking through a microscope can inspire, applying the technology to our own research questions can be intimidating. Like any field, optical microscopy has its own language, jargon, and conceptual framework, but is rarely taught in academic programs. To learn...