Microscopy Field Notes
A blog created to help life science researchers navigate the inspiring world of optical microscopy
Connecting the Dots: Laser Scanning in Confocal and Multiphoton Microscopes
Taking the time to understand the inner workings of an imaging system can help you design more robust experiments and acquire higher-quality data. It can also help you trouble-shoot when a system is not performing as expected. Over the next few posts, we will...
Powering Through It: Imaging Deep in Scattering Media
In the last post, we reviewed how scattering and absorption attenuate the intensity of the laser used to excite fluorescence and reduce the amount of emission signal that can make it to the detector(s). Tissue is very complex, and fluorescence from different dyes, as...
Friends and Foes: Scattering and Absorption
When carrying out fluorescence imaging in thicker samples, we often hear about how “scattering” affects image quality by limiting the depth of imaging within the sample. Scattering occurs when light traveling through a sample encounters a change in refractive index, ...
Highlighting Color Correction
Often we want to visualize multiple structures within the same preparation, and if the colors are not registered, this can lead to erroneous conclusions. For example, if you are counting nuclei, the color correction does not likely matter, but when examining whether a...
Making Sense of the Fine Details
Over the next few posts, we will dig deeper into microscope objectives and explore how they can affect the quality of your imaging. Let’s start the series off with magnification and resolution, which can be a point of confusion when planning experiments. Often,...
Photons to Pixels: Illuminating Bit Depth
The meaning and relevance of bit depth is a common point of confusion for life scientists who use microscopes. Typically, when we mention bit depth in microscopy, we are referring to gradations in the intensity readings from a detector. Let’s use a photomultiplier...