Microscopy Field Notes

A blog created to help life science researchers navigate the inspiring world of optical microscopy

Friends and Foes: Scattering and Absorption

Friends and Foes: Scattering and Absorption

When carrying out fluorescence imaging in thicker samples, we often hear about how  “scattering” affects image quality by limiting the depth of imaging within the sample. Scattering occurs when light traveling through a sample encounters a change in refractive index, ...

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Highlighting Color Correction

Highlighting Color Correction

Often we want to visualize multiple structures within the same preparation, and if the colors are not registered, this can lead to erroneous conclusions. For example, if you are counting nuclei, the color correction does not likely matter, but when examining whether a...

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Making Sense of the Fine Details

Making Sense of the Fine Details

Over the next few posts, we will dig deeper into microscope objectives and explore how they can affect the quality of your imaging. Let’s start the series off with magnification and resolution, which can be a point of confusion when planning experiments.    Often,...

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Photons to Pixels: Illuminating Bit Depth

Photons to Pixels: Illuminating Bit Depth

The meaning and relevance of bit depth is a common point of confusion for life scientists who use microscopes. Typically, when we mention bit depth in microscopy, we are referring to gradations in the intensity readings from a detector.  Let’s use a photomultiplier...

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