Microscopy Field Notes

In our previous blog post we discussed resonant and polygonal mirror scanning systems. These scanning systems are designed for high-speed imaging, and can achieve short pixel dwell times compared to conventional galvanometer mirrors. While short pixel dwell times...

In our previous post, we discussed galvo scanning systems used in laser scanning confocal and multiphoton microscopes. Galvo scanning is standard, yet for some applications, such as live-cell and large-format image acquisitions, systems that offer increased...

Taking the time to understand the inner workings of an imaging system can help you design more robust experiments and acquire higher-quality data. It can also help you trouble-shoot when a system is not performing as expected. Over the next few posts, we will...

In the last post, we reviewed how scattering and absorption attenuate the intensity of the laser used to excite fluorescence and reduce the amount of emission signal that can make it to the detector(s). Tissue is very complex, and fluorescence from different dyes, as...

When carrying out fluorescence imaging in thicker samples, we often hear about how  “scattering” affects image quality by limiting the depth of imaging within the sample. Scattering occurs when light traveling through a sample encounters a change in refractive index, ...

Often we want to visualize multiple structures within the same preparation, and if the colors are not registered, this can lead to erroneous conclusions. For example, if you are counting nuclei, the color correction does not likely matter, but when examining whether a...