Pina Colarusso

Pina has been working as an optical microscopist over the past twenty years. Currently she directs an imaging centre, the Snyder Institute’s Live Imaging Laboratory (LCI) at the University of Calgary.

Author articles

Making Sense of the Fine Details

Making Sense of the Fine Details

Over the next few posts, we will dig deeper into microscope objectives and explore how they can affect the quality of your imaging. Let’s start the series off with magnification and resolution, which can be a point of confusion when planning experiments.    Often,...

Photons to Pixels: Illuminating Bit Depth

Photons to Pixels: Illuminating Bit Depth

The meaning and relevance of bit depth is a common point of confusion for life scientists who use microscopes. Typically, when we mention bit depth in microscopy, we are referring to gradations in the intensity readings from a detector.  Let’s use a photomultiplier...

Two-Photon Microscopy: How to Minimize Optical Aberrations

Two-Photon Microscopy: How to Minimize Optical Aberrations

The main advantage of two-photon microscopy is the ability to image deeper into scattering samples, such as tissue and live specimens. One way that two-photon objectives assist deep imaging is by providing extended working distances compared to lenses for other...

Two-Photon Microscopy Objectives: Focusing on the Essentials

Two-Photon Microscopy Objectives: Focusing on the Essentials

In our last post, Craig and I introduced the basics of two-photon microscopy. This month we will review some considerations for choosing the best objective for your multiphoton imaging. If you are new to objectives and their properties, we recommend that you consult...

What Is Two-Photon Microscopy?

What Is Two-Photon Microscopy?

This week I am joined by Dr. Craig Brideau, an engineering scientist who has recently joined the LCI team. Craig has designed and built numerous optical systems, and has particular expertise in biomedical imaging using non-linear optics. Over the next several posts,...

Honouring Maria Goeppert-Mayer for International Women’s Day

Honouring Maria Goeppert-Mayer for International Women’s Day

In recognition of International Women’s Day, I would like to highlight the German-American physicist Maria Goeppert-Mayer (1906-1972). Goeppert-Mayer started off in mathematics but was inspired to switch to physics after attending a quantum mechanics seminar presented...

Multicolour Imaging: Moving From Theory to Practice

Multicolour Imaging: Moving From Theory to Practice

Multicolour fluorescence imaging, when rigorously applied, allows the selective visualization of two or more features within a sample. The qualification “when rigorously applied” is so important because multicolour imaging is prone to artifacts. For multicolour...

Decoding and Demystifying Filters

Decoding and Demystifying Filters

Developing expertise in microscopy, like for any technical field, requires mastering fundamental concepts and skills while also developing fluency in the specific jargon. Although jargon can be convenient for facilitating communication, it often creates barriers for...

The Path Is Made by Walking

The Path Is Made by Walking

This month we share some reflections from Grace Baruta, who is a PhD candidate in the Hirota Laboratory at the University of Calgary. I have known Grace since she was a summer student, and she has always taken the time to research options and map out an approach, from...

The Sample Sets the Stage

The Sample Sets the Stage

So far, we’ve touched on the mindset, planning, and decisions required when carrying out imaging experiments. To highlight this framework with a real-life example, I have invited Louise Neave to share some of her experiences as a researcher new to imaging. Louise is a...

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