Pina Colarusso and Craig Brideau

Pina has been working as an optical microscopist over the past twenty years. Currently she directs an imaging centre, the Snyder Institute’s Live Imaging Laboratory (LCI) at the University of Calgary.

Author articles

Powering Through It: Imaging Deep in Scattering Media

Powering Through It: Imaging Deep in Scattering Media

In the last post, we reviewed how scattering and absorption attenuate the intensity of the laser used to excite fluorescence and reduce the amount of emission signal that can make it to the detector(s). Tissue is very complex, and fluorescence from different dyes, as...

Friends and Foes: Scattering and Absorption

Friends and Foes: Scattering and Absorption

When carrying out fluorescence imaging in thicker samples, we often hear about how  “scattering” affects image quality by limiting the depth of imaging within the sample. Scattering occurs when light traveling through a sample encounters a change in refractive index, ...

Highlighting Color Correction

Highlighting Color Correction

Often we want to visualize multiple structures within the same preparation, and if the colors are not registered, this can lead to erroneous conclusions. For example, if you are counting nuclei, the color correction does not likely matter, but when examining whether a...

Making Sense of the Fine Details

Making Sense of the Fine Details

Over the next few posts, we will dig deeper into microscope objectives and explore how they can affect the quality of your imaging. Let’s start the series off with magnification and resolution, which can be a point of confusion when planning experiments.    Often,...

Photons to Pixels: Illuminating Bit Depth

Photons to Pixels: Illuminating Bit Depth

The meaning and relevance of bit depth is a common point of confusion for life scientists who use microscopes. Typically, when we mention bit depth in microscopy, we are referring to gradations in the intensity readings from a detector.  Let’s use a photomultiplier...

Two-Photon Microscopy: How to Minimize Optical Aberrations

Two-Photon Microscopy: How to Minimize Optical Aberrations

The main advantage of two-photon microscopy is the ability to image deeper into scattering samples, such as tissue and live specimens. One way that two-photon objectives assist deep imaging is by providing extended working distances compared to lenses for other...

Two-Photon Microscopy Objectives: Focusing on the Essentials

Two-Photon Microscopy Objectives: Focusing on the Essentials

In our last post, Craig and I introduced the basics of two-photon microscopy. This month we will review some considerations for choosing the best objective for your multiphoton imaging. If you are new to objectives and their properties, we recommend that you consult...

What Is Two-Photon Microscopy?

What Is Two-Photon Microscopy?

This week I am joined by Dr. Craig Brideau, an engineering scientist who has recently joined the LCI team. Craig has designed and built numerous optical systems, and has particular expertise in biomedical imaging using non-linear optics. Over the next several posts,...

Honouring Maria Goeppert-Mayer for International Women’s Day

Honouring Maria Goeppert-Mayer for International Women’s Day

In recognition of International Women’s Day, I would like to highlight the German-American physicist Maria Goeppert-Mayer (1906-1972). Goeppert-Mayer started off in mathematics but was inspired to switch to physics after attending a quantum mechanics seminar presented...

Multicolour Imaging: Moving From Theory to Practice

Multicolour Imaging: Moving From Theory to Practice

Multicolour fluorescence imaging, when rigorously applied, allows the selective visualization of two or more features within a sample. The qualification “when rigorously applied” is so important because multicolour imaging is prone to artifacts. For multicolour...

Subscribe to the blog