by Pina Colarusso and Craig Brideau | Nov 23, 2022 | Uncategorized
When carrying out fluorescence imaging in thicker samples, we often hear about how “scattering” affects image quality by limiting the depth of imaging within the sample. Scattering occurs when light traveling through a sample encounters a change in refractive index, ...Applications | Features | Benefits |
Fixed cell imaging | - Multi-channel acquisition | SPARQ can be used with any fluorophore |
Live cell imaging | - Low laser intensity (comparable to standard widefield illumination) | Reduces photobleaching and phototoxity, allowing for long imaging sessions |
| - Fast 2D and 3D imaging | With a frame rate of up to 10 FPS, catch your dynamic proccesses using SPARQ | |
| - Multi-dimensional acquisition: Time lapse, z-stacks, multi-channels | Set your usual multi-dimensional experiments and turn on SPARQ for optically sectioned images | |
| - Combinaison with transmitted light constrat methods | Combine your SPARQ images with any contrast modes, without even changing objectives | |
Tissue slice imaging | - High penetration depth | Since the speckles remain sharp in depth, get the same optical sectioning capabilities as you go deeper |
| - Modification of optical thickness | Adjust SPARQ's thickness factor parameter to change the axial resolution without affecting the lateral resolution | |
| - 3D imaging and reconstruction | Create high quality 3D images by rejecting out-of-focus fluorescence | |
Whole mount and cleared tissue imaging | - Large area stitching | Keep the same imaging speed regardless of the field of view. SPARQ works with any camera |
| - Use different objective magnifications | Simply change objectives without the need to re-calibrate or to modify the SPARQ parameter | |
| - Works with any kind of objectives | Use high N.A, LWD or phase contrast objectives. It will not affect the SPARQ images | |
| - Keep high axial and lateral resolution | Improve the optical sectioning without sacrificing the lateral resolution as with standard confocal microscopy | |
In vivo imaging (on 2P) | - Can be used in combinaison with photo-stimulation | Use the same visible lasers to photo-stimulate and do SPARQ imaging (different light paths) |
| - Offers optical sectioning with visible lasers on a 2P optimized system | Increase the flexibilty of your 2P system by adding SPARQ and get visible light confocality | |
| - Can be used in vivo for positioning and other fluorescence uses | A one push button allows you to easily switch between SPARQ and other imaging modes |
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