by Pina Colarusso and Craig Brideau | Oct 28, 2021 | Uncategorized
So far, we’ve touched on the mindset, planning, and decisions required when carrying out imaging experiments. To highlight this framework with a real-life example, I have invited Louise Neave to share some of her experiences as a researcher new to imaging. Louise is a...Applications | Features | Benefits |
Fixed cell imaging | - Multi-channel acquisition | SPARQ can be used with any fluorophore |
Live cell imaging | - Low laser intensity (comparable to standard widefield illumination) | Reduces photobleaching and phototoxity, allowing for long imaging sessions |
| - Fast 2D and 3D imaging | With a frame rate of up to 10 FPS, catch your dynamic proccesses using SPARQ | |
| - Multi-dimensional acquisition: Time lapse, z-stacks, multi-channels | Set your usual multi-dimensional experiments and turn on SPARQ for optically sectioned images | |
| - Combinaison with transmitted light constrat methods | Combine your SPARQ images with any contrast modes, without even changing objectives | |
Tissue slice imaging | - High penetration depth | Since the speckles remain sharp in depth, get the same optical sectioning capabilities as you go deeper |
| - Modification of optical thickness | Adjust SPARQ's thickness factor parameter to change the axial resolution without affecting the lateral resolution | |
| - 3D imaging and reconstruction | Create high quality 3D images by rejecting out-of-focus fluorescence | |
Whole mount and cleared tissue imaging | - Large area stitching | Keep the same imaging speed regardless of the field of view. SPARQ works with any camera |
| - Use different objective magnifications | Simply change objectives without the need to re-calibrate or to modify the SPARQ parameter | |
| - Works with any kind of objectives | Use high N.A, LWD or phase contrast objectives. It will not affect the SPARQ images | |
| - Keep high axial and lateral resolution | Improve the optical sectioning without sacrificing the lateral resolution as with standard confocal microscopy | |
In vivo imaging (on 2P) | - Can be used in combinaison with photo-stimulation | Use the same visible lasers to photo-stimulate and do SPARQ imaging (different light paths) |
| - Offers optical sectioning with visible lasers on a 2P optimized system | Increase the flexibilty of your 2P system by adding SPARQ and get visible light confocality | |
| - Can be used in vivo for positioning and other fluorescence uses | A one push button allows you to easily switch between SPARQ and other imaging modes |
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